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Cytokine influence on simian immunodeficiency virus replication within primary macrophages. TNF-alpha, but not GMCSF, enhances viral replication on a per-cell basis.
Walsh DG; Horvath CJ; Hansen-Moosa A; MacKey JJ; Sehgal PK; Daniel MD;
January 30, 1992
Am J Pathol. 1991 Oct;139(4):877-87. Unique Identifier : AIDSLINE

The control of HIV-1 or SIV replication within macrophages is probably influenced by a variety of viral and cellular factors. Of the cellular factors, the authors have studied cytokine influence on SIV replication in vitro utilizing simian alveolar macrophages and uncloned SIVmacMTV, a macrophage-tropic variant. The approach allowed quantification of viral replication on a per-cell basis. As reported for HIV-1 replication in macrophages, TNF-alpha significantly increased SIV production in these macrophage cultures. GMCSF also resulted in marked increases in SIV gag protein in culture supernatants. However, after correcting for differences in total cell numbers and numbers of gag-containing cells in the treated and untreated cultures, GMCSF did not upregulate SIV production on a per-cell basis. IL-6 increased SIV replication little if at all but induced significantly greater cytopathic changes in the treated cultures compared with infected, untreated cultures. In contrast, IFN-gamma greatly decreased replication. Our results for GMCSF, IFN-gamma, and IL-6 are in contrast to previously published reports of cytokine control of HIV-1 growth in target cells, and they stress the importance of cell number analyses and the use of primary cultures in the study of lentiviral replication kinetics in macrophages.

Animal Bronchoalveolar Lavage Fluid/CYTOLOGY Cells, Cultured Cytokines/*PHARMACOLOGY Cytoplasm/DRUG EFFECTS/MICROBIOLOGY Granulocyte-Macrophage Colony-Stimulating Factor/*PHARMACOLOGY Interferon Type II/PHARMACOLOGY Interleukin-6/PHARMACOLOGY Macaca mulatta Macrophages/*MICROBIOLOGY Support, U.S. Gov't, P.H.S. SIV/*PHYSIOLOGY Tumor Necrosis Factor/*PHARMACOLOGY Up-Regulation (Physiology)/DRUG EFFECTS Virus Replication/*DRUG EFFECTS JOURNAL ARTICLE