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Identification of the primer binding domain in human immunodeficiency virus reverse transcriptase.
Basu A; Ahluwalia KK; Basu S; Modak MJ; Department of Biochemistry and
April 30, 1992
Biochemistry. 1992 Jan 21;31(2):616-23. Unique Identifier : AIDSLINE

We have labeled the primer binding domain of HIV1-RT with 5'-32P-labeled (dT)15 primer using ultraviolet light energy. The specificity of the primer cross-linking to HIV1-RT was demonstrated by competition experiments. Both synthetic and natural primers, e.g., p(dA)15, p(dC)15, and tRNA(Lys), inhibit p(dT)15 binding and cross-linking to the enzyme. The observed binding and cross-linking of the primer to the enzyme were further shown to be functionally significant by the observation that tRNA(Lys) inhibits the polymerase activity on poly(rA).(dT)15 template-primer as well as the cross-linking of p(dT)15 to the enzyme to a similar extent. At an enzyme to p(dT)15 ratio of 1:3, about 15% of the enzyme can be cross-linked to the primer. To identify the domain cross-linked to (dT)15, tryptic peptides were generated and purified by a combination of HPLC on a C-18 reverse-phase column and DEAE-Sephadex chromatography. A single peptide cross-linked to p(dT)15 was identified. This peptide corresponded to amino acid residues 288-307 in the primary sequence of HIV1-RT as judged by amino acid composition and sequence analyses. Further, Leu(289)-Thr(290) and Leu(295)-Thr(296) of HIV1-RT appear to be the probable sites of cross-linking to the primer p(dT)15.

Amino Acid Sequence Chromatography, DEAE-Cellulose Chromatography, High Pressure Liquid Cross-Linking Reagents HIV-1/*ENZYMOLOGY Molecular Sequence Data Oligodeoxyribonucleotides/*CHEMISTRY Protein Binding Protein Conformation RNA-Directed DNA Polymerase/*ANALYSIS/CHEMISTRY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Templates JOURNAL ARTICLE