translation agency

NLM AIDSLINE
Specific NF-kappa B subunits act in concert with Tat to stimulate human immunodeficiency virus type 1 transcription.
Liu J; Perkins ND; Schmid RM; Nabel GJ; Department of Internal Medicine,
August 30, 1992
J Virol. 1992 Jun;66(6):3883-7. Unique Identifier : AIDSLINE

NF-kappa B is a protein complex which functions in concert with the tat-I gene product to stimulate human immunodeficiency virus (HIV) transcription. To determine whether specific members of the NF-kappa B family contribute to this effect, we have examined the abilities of different NF-kappa B subunits to act with Tat-I to stimulate transcription of HIV in Jurkat T-leukemia cells. We have found that the p49(100) DNA binding subunit, together with p65, can act in concert with Tat-I to stimulate the expression of HIV-CAT plasmid. Little effect was observed with 50-kDa forms of p105 NF-kappa B or rel, in combination with p65 or full-length c-rel, which do not stimulate the HIV enhancer in these cells. These findings suggest that the combination of p49(100) and p65 NF-kappa B can act in concert with the tat-I gene product to stimulate the synthesis of HIV RNA.

Cells, Cultured Chloramphenicol Acetyltransferase/BIOSYNTHESIS/GENETICS Gene Products, tat/*METABOLISM Human HIV-1/*METABOLISM NF-kappa B/CLASSIFICATION/*METABOLISM Retroviridae Proteins, Oncogenic/METABOLISM Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. T-Lymphocytes/*METABOLISM Trans-Activation (Genetics) *Transcription, Genetic Transfection JOURNAL ARTICLE

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