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Human immunodeficiency virus type 2 infection and fusion of CD4-negative human cell lines: induction and enhancement by soluble CD4.
Clapham PR; McKnight A; Weiss RA; Chester Beatty Laboratories, Institute
August 30, 1992
J Virol. 1992 Jun;66(6):3531-7. Unique Identifier : AIDSLINE

We describe human immunodeficiency type 2 (HIV-2) strains which induce cell-to-cell fusion and infect certain CD4- human cell lines. Soluble CD4 (sCD4) induces or enhances fusion by most HIV-2 strains tested. Soluble CD4-immunoglobulin G chimeras and conjugates of sCD4 and antibody to the third domain of CD4 block HIV-2 fusion of CD4- cells. We conclude that HIV-2 can enter CD4- cells via an alternative cell surface receptor to CD4. While some strains entered efficiently, others retained a dependency on an interaction with sCD4 to initiate changes in the virion envelope required for membrane fusion.

Acquired Immunodeficiency Syndrome/*MICROBIOLOGY Antigens, CD4/*METABOLISM Cell Fusion/DRUG EFFECTS/*PHYSIOLOGY Cell Line DNA Mutational Analysis Human HIV Envelope Protein gp120/METABOLISM HIV-2/*PATHOGENICITY Receptors, HIV/METABOLISM Recombinant Fusion Proteins/METABOLISM Rhabdomyosarcoma/MICROBIOLOGY Support, Non-U.S. Gov't Tumor Cells, Cultured JOURNAL ARTICLE