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Antibody responses to a major Pneumocystis carinii antigen in human immunodeficiency virus-infected patients with and without P. carinii pneumonia.
Lundgren B; Lundgren JD; Nielsen T; Mathiesen L; Nielsen JO; Kovacs JA;
August 30, 1992
J Infect Dis. 1992 Jun;165(6):1151-5. Unique Identifier : AIDSLINE

Antibody responses to a major purified human Pneumocystis carinii surface antigen (gp95) were determined by ELISA in human immunodeficiency virus (HIV)-infected patients. Serum IgG directed against gp95 was measured in 129 consecutive HIV-infected patients who underwent bronchoscopy for evaluation of pulmonary symptoms. Significantly more patients with P. carinii pneumonia (PCP) had detectable antibodies compared with HIV-infected patients without PCP and with HIV-negative controls (50 [66%] of 76 vs. 18 [34%] of 53 and 7 [35%] of 20, respectively; P less than .001), and the level of antibody response was higher (mean optical density ratio: 0.6 vs. 0.23 and 0.2, respectively; P less than .01). Changes in antibody response were investigated in 78 patients for whom serial serum samples taken around the time of bronchoscopy were available. Of the 47 patients with verified PCP, 20 (43%) mounted an antibody response, compared with only 1 (3%) of 31 patients without PCP (P less than .001). This patient had PCP on the basis of clinical criteria, including response to therapy. Thus, despite severe immunosuppression, a proportion of HIV-infected patients with PCP can mount a specific IgG-mediated antibody response to P. carinii.

Antibodies, Fungal/*BIOSYNTHESIS/BLOOD Antigens, Fungal/*IMMUNOLOGY Antigens, Surface/IMMUNOLOGY Blotting, Western Enzyme-Linked Immunosorbent Assay Human HIV Infections/*COMPLICATIONS/IMMUNOLOGY IgG/BIOSYNTHESIS/BLOOD Pneumocystis carinii/*IMMUNOLOGY Pneumonia, Pneumocystis carinii/*COMPLICATIONS/DIAGNOSIS/ IMMUNOLOGY Predictive Value of Tests Sensitivity and Specificity Support, Non-U.S. Gov't JOURNAL ARTICLE

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