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Interactions of CD4+ plasma membrane vesicles with HIV-1 and HIV-1 envelope glycoprotein-expressing cells.
Puri A; Dimitrov DS; Golding H; Blumenthal R; Section on Membrane
December 30, 1992
J Acquir Immune Defic Syndr. 1992;5(9):915-20. Unique Identifier :

To study interactions between the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (gp120-gp41) and the receptor in the target membrane, CD4, a new experimental system utilizing CD4-carrying plasma membrane vesicles (CD4 PMVs) was developed. CD4 PMVs were prepared by hypotonic lysis of HeLa cells expressing CD4 after infection with recombinant vaccinia virus containing the CD4 cDNA. The CD4 PMVs carried up to 680 CD4 molecules per vesicle. Their fusion with cells expressing gp120-gp41 after infection with recombinant vaccinia virus was monitored by fluorescence video microscopy by using lipophilic fluorescent dyes. Fluorescence changes as a result of fusion occurred within 30 min at 37 degrees C, and little fluorescence changes were seen with cells expressing the noncleaved HIV-1 envelope glycoprotein (gp160). The preincubation of CD4 PMVs with HIV-1 reduced its infectivity 10-fold. The CD4 PMVs were more effective in inhibiting syncytia formation than sCD4. These results demonstrate that CD4 PMVs could be used to study the mechanisms of HIV-1 envelope-mediated fusion and have the potential to inactivate HIV-1.

Animal Antigens, CD4/*METABOLISM Cell Line Cell Membrane/*METABOLISM Hela Cells Human HIV Envelope Protein gp120/*METABOLISM HIV Envelope Protein gp41/*METABOLISM HIV-1/*METABOLISM Microscopy, Fluorescence Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE

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