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Inverse association of IgG-anti-Fab antibodies and CD4 cell counts in patients with AIDS/ARC.
Susal C; Daniel V; Moravedji M; Jager H; Ujhelyi E; Huth-Kuhne A; Doerr
December 30, 1992
Int Conf AIDS. 1992 Jul 19-24;8(3):39 (abstract no. PuA 6168). Unique

OBJECTIVE: To investigate whether anti-Fab autoantibodies that have a protective effect on kidney graft outcome and possess immunosuppressive properties play a role in the immunopathogenesis of AIDS. METHODS: Sera of HIV+ hemophilia patients and homosexual men with AIDS/ARC, without AIDS/ARC, HIV- hemophilia patients, and healthy controls (n = 542) were tested in specific ELISA assays for IgG-, IgA-, and IgM-anti-Fab activities. RESULTS: We found a striking inverse association between IgG- or IgA-anti-Fab and CD4+ cell counts (r = -0.7 or 0,4, p less than 10(-4)) in hemophilia patients. Follow up (34 +/- 1 months) in 16 HIV+ hemophilia patients with AIDS/ARC showed that every clear increase of IgG-anti-Fab correlated with a decrease of CD4+ cell counts. Similar results were obtained in HIV+ homosexual men. Antibody of an AIDS patient affinity purified on a human IgG, Fab2-coated column bound to Fab, rgp120, rgp120-coated resting CD4+ cells, and to a B-CLL clone (IgM+). No binding was observed to sCD4 or CD4+ cells from peripheral blood. Weaker binding was found to a CD4-/CD8-/MHC class II+ clone suggesting cross-reactivity with MHC II or another antigen on activated T cells. Addition of the affinity purified antibody and complement to rgp120-coated CD4+ cells resulted in lysis of uninfected CD4+ cells. CONCLUSION: Our data indicate that anti-Fab antibodies may contribute to the elimination of CD4+ cells in AIDS.

Acquired Immunodeficiency Syndrome/BLOOD/*IMMUNOLOGY Antibodies, Anti-Idiotypic/*BLOOD Antigens, CD4/*ANALYSIS AIDS-Related Complex/BLOOD/*IMMUNOLOGY Hemophilia/BLOOD/COMPLICATIONS/IMMUNOLOGY Homosexuality Human HIV Antibodies/BLOOD/*IMMUNOLOGY HIV Seropositivity IgA/IMMUNOLOGY IgG/*IMMUNOLOGY Immunoglobulins, Fab/*IMMUNOLOGY Male Reference Values T-Lymphocyte Subsets/*IMMUNOLOGY ABSTRACT