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Vitamin D3 compounds regulate human immunodeficiency virus type 1 replication in U937 monoblastoid cells and in monocyte-derived macrophages.
Pauza CD; Kornbluth R; Emau P; Richman DD; Deftos LJ; Department of
June 30, 1993
J Leukoc Biol. 1993 Feb;53(2):157-64. Unique Identifier : AIDSLINE

We studied the effects of vitamin D3 compounds on the replication of human immunodeficiency virus type 1 (HIV-1) in the monoblastoid cell line U937 and in primary monocyte-derived macrophage cultures to understand how modulators of monocyte/macrophage effector function might affect the pathogenesis of HIV-1 infection. U937 cell cultures exposed to 1, alpha 25-dihydroxyvitamin D3 prior to HIV-1 infection showed enhanced virus replication that was apparently due to increased cellular resistance to viral cytopathic effects; a marked inhibition of virus replication was noted in cells exposed to 1 alpha,25-dihydroxyvitamin D3 subsequent to infection. Exposure of blood-derived monocyte/macrophages to vitamin D3 compounds prior to infection also affected virus growth; in most cases, substantial inhibition of HIV-1 replication was noted in vitamin D3-treated macrophage cultures. Our results demonstrate that vitamin D3 compounds with recognized abilities to induce cellular differentiation can modulate HIV-1 infection of human macrophages.

Calcifediol/PHARMACOLOGY Calcitriol/PHARMACOLOGY Cells, Cultured Cholecalciferol/*PHARMACOLOGY Human HIV Seropositivity HIV-1/DRUG EFFECTS/*PHYSIOLOGY Kinetics Macrophages/DRUG EFFECTS/*PHYSIOLOGY Monocytes/DRUG EFFECTS/*PHYSIOLOGY Structure-Activity Relationship Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Tumor Cells, Cultured Virus Replication/*DRUG EFFECTS JOURNAL ARTICLE

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