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Recombinant immunoblot assays for hepatitis C in human immunodeficiency virus type 1-infected US Navy personnel.
Bryan JP; Sjogren MH; Malone JL; MacArthy P; Kao TC; Wagner K; Sheffield
June 30, 1993
J Infect Dis. 1993 Mar;167(3):715-9. Unique Identifier : AIDSLINE

The prevalence of hepatitis A, B, C, and D viruses was studied in 467 military personnel with human immunodeficiency virus type 1 (HIV-1) infection. Antibody to hepatitis C virus (anti-HCV) by first-generation ELISA was found in 136 (29%). Of sera repeatedly reactive for anti-HCV by first-generation ELISA, two-antigen recombinant immunoblot assay (RIBA) was positive in 41 (32%) and four-antigen RIBA was positive in 55 (41%). Four-antigen RIBA was positive in 33 (30%) of the 109 with an OD on ELISA of < or = 2.0 compared with 22 (81%) of the 27 with an OD > 2.0 (P < .001). Anti-HCV detected by four-antigen RIBA was associated with increasing age, black or Hispanic race, and antibody to hepatitis B core antigen. When patients with hepatitis B surface antigen were excluded, elevated alanine aminotransferase was found in 5 (8%) of 63 with a negative RIBA and 13 (28%) of 47 with a positive RIBA (P = .006). While RIBA was negative in more than half of those with anti-HCV by ELISA, 55 (12%) of these HIV-1 infected personnel had anti-HCV detected by RIBA, which was associated with a strong reaction by ELISA, elevated liver enzymes, coinfection with hepatitis B, minority race, and older age.

Alanine Aminotransferase/BLOOD Analysis of Variance *AIDS-Related Opportunistic Infections Enzyme-Linked Immunosorbent Assay Female Hepatitis Antibodies/BLOOD Hepatitis B Surface Antigens/BLOOD Hepatitis C/COMPLICATIONS/DIAGNOSIS/*EPIDEMIOLOGY Hepatitis C Viruses/IMMUNOLOGY Hepatitis, Viral, Human/EPIDEMIOLOGY Human *HIV-1 Immunoblotting/METHODS Male *Military Personnel Naval Medicine Regression Analysis Sex Factors Support, U.S. Gov't, Non-P.H.S. Syphilis/COMPLICATIONS United States JOURNAL ARTICLE