J Clin Microbiol. 1993 Mar;31(3):681-4. Unique Identifier : AIDSLINE
Early diagnosis of human immunodeficiency virus (HIV) infection may be
difficult in adults with acute or recent HIV infection and in infants
with perinatally acquired HIV. Detection of HIV-specific immunoglobulin
A (IgA) antibodies in infant serum by Western blot (immunoblot) has been
suggested as a reliable method to identify HIV-infected infants,
especially those over the age of 6 months, and as an adjunct to
diagnosis of acute HIV infection in adults. We developed a simple enzyme
immunoassay for detection of HIV-specific IgA, using standard
commercially available reagents. Enzyme immunoassay was comparable to
Western blot for detection of HIV-specific IgA in sera from adults (n =
216), older children (n = 49), and infants born to HIV-infected mothers
(n = 65). Specificity was 100% and sensitivity ranged from 80 to 92%.
IgA-enzyme immunoassay is a simple, highly sensitive method for
detection of HIV-specific IgA antibodies and is easily adapted to the
standard clinical laboratory.
Adolescence Adult Aged Antibody Specificity Blotting, Western Child
Child, Preschool Comparative Study Female Finland/EPIDEMIOLOGY Human
HIV Antibodies/*BLOOD HIV
IgA/*ANALYSIS *Immunoenzyme Techniques Infant Male Middle Age
Predictive Value of Tests Sensitivity and Specificity Support, U.S.
Gov't, P.H.S. JOURNAL ARTICLE