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A novel monoclonal antibody specifically reactive with human T-lymphotropic virus type-II (HTLV-II) envelope protein.
Ohara N; Hayashi K; Takahashi K; Miyamoto K; Akagi T; Second Department
October 30, 1993
Int J Cancer. 1993 Jul 9;54(5):858-61. Unique Identifier : AIDSLINE

A novel monoclonal antibody (MAb), N5.4.4, specifically reactive with human T-lymphotropic virus type-II (HTLV-II) envelope glycoprotein (gp) has been developed through immunization with a synthetic peptide corresponding to the amino acid sequence 171-196 of the HTLV-II envelope protein (II-env 171-196). This MAb, which belonged to the IgG1 kappa subclass, reacted with the cytosmears of HTLV-II-infected cell lines (Si-IIA, CR-IIA-I and AS-IIA), but not with those of HTLV-I-infected cell lines (MT-1 and MT-2) or other HTLV-uninfected cell lines. On Western blot analysis, this MAb reacted with gp46 of HTLV-II lysates but not with HTLV-1 lysates. Moreover, flow-cytometric analysis revealed that this MAb recognized the native surface of only the HTLV-II-producing cells. Through application to immunohistochemical or serological method, this MAb may be of value in elucidating the pathogenesis of HTLV-II infection in comparison with HTLV-I.

Amino Acid Sequence Animal Antibodies, Monoclonal/*IMMUNOLOGY Antibody Specificity/*IMMUNOLOGY Blotting, Western Flow Cytometry Human HTLV-II Antigens/CHEMISTRY/*IMMUNOLOGY Immunoglobulins, kappa-Chain/IMMUNOLOGY Mice Mice, Inbred BALB C Molecular Sequence Data Viral Envelope Proteins/CHEMISTRY/*IMMUNOLOGY JOURNAL ARTICLE