Kansenshogaku Zasshi. 1993 May;67(5):421-8. Unique Identifier : AIDSLINE
We have demonstrated the nested-polymerase chain reaction (nested-PCR)
assay detected from 14 mycobacterial species. We have further
demonstrated the species specific restriction sites within amplified
dnaJ gene, which allowed us to differentiate the mycobacterial DNA by
combination of the PCR with the restriction fragment length polymorphism
(PCR-RFLP). Nested-PCR-RFLP was used to detect and identified
mycobacterial DNA in the sputa samples and HIV related samples. The
target DNA was a 196-base pair segment of dnaJ gene. Of 68 sputum
samples tested, 7 were smear positive for acid-fast bacilli and culture.
The 7 samples were also identified with Mycobacterium tuberculosis by
PCR-RFLP. We tested 69 HIV related samples (19 frozen samples, 22
paraffin embedded samples and 28 lymphocyte from HIV infected people).
Of 38 samples were positive for nested-PCR, 8 M. tuberculosis complex, 7
M. avium, 8 M. intracellulare, and 16 others were detected by PCR-RFLP.
The PCR method is useful for the rapid diagnosis of mycobacterial
Acquired Immunodeficiency Syndrome/*MICROBIOLOGY AIDS-Related
Opportunistic Infections/DIAGNOSIS Base Sequence DNA,
Bacterial/*ANALYSIS English Abstract Human Molecular Sequence Data
Mycobacterium/GENETICS/*ISOLATION & PURIF Polymerase Chain
Reaction/METHODS Sputum/*CHEMISTRY Support, Non-U.S. Gov't JOURNAL