Int Conf AIDS. 1993 Jun 6-11;9(1):382 (abstract no. PO-B10-1480). Unique
OBJECTIVES: To describe the detection of repetitive DNA sequences of
Toxoplasma gondii in blood samples from AIDS patients with suspected
toxoplasmic encephalitis. METHODS: Blood samples were collected from 12
individuals: Four of them with a presumptive diagnosis of toxoplasmosis.
One with a confirmed diagnosis of cerebral tuberculosis (TB) and the
seven left were taken as healthy controls. Cerebral spinal fluids (CSF)
samples were obtained from the same patients evaluated for central
nervous system disorders. DNA extracted from the buffy coat of the blood
samples and from CSF samples were hybridized with ABGTg4 DNA probe as it
was described previously (Angel, S. J. Clin. Microbiol., dec 1992, 30).
Tests were performed by triplicates. RESULTS: Both blood and CSF samples
were positive in the four patients that showed clinical, tomographic and
biopsy evidences of CNS toxoplasmosis and they were negative in the
patient that presented cerebral TB. Blood samples from healthy controls
were all negative, confirming the specificity of the test. CONCLUSIONS:
We described here a non invasive technique (ABGTg4 dot blot
hybridization in blood samples) for confirming a presumptive diagnosis
of toxoplasmosis in ADS patients. We are currently evaluating this
technique for the early detection of disseminated toxoplasmosis in
patients at risk.
*AIDS-Related Opportunistic Infections/DIAGNOSIS *DNA, Protozoan/BLOOD
*Toxoplasma/ISOLATION & PURIF *Toxoplasmosis/DIAGNOSIS