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Activation associated lymphocyte death (AALD) in HIV-1+ patients is associated with the appearance of CD38+CD45RO+ and CD28- lymphocytes.
Borthwick N; Bofill M; Medina E; Gombert W; Janossy G; Department of
November 30, 1993
Int Conf AIDS. 1993 Jun 6-11;9(1):32 (abstract no. WS-A16-1). Unique

After in vitro stimulation lymphocytes from HIV-1 infected patients undergo activation associated lymphocyte (AALD) as shown by a decrease in the number of lymphoblasts recovered. In this study we have more closely investigated this phenomenon in relation to the atypical phenotypic features of lymphocytes from HIV-1+ patients. There was an overall increase in the number of CD38+ lymphocytes in patients due to an accumulation of cells with a novel CD8+CD38+CD45RO+ phenotype. This correlated negatively with the number of surviving blasts after PHA activation (r = -0.505, p < 0.001). This phenotype was also found to correlate well with other markers of disease progression such as serum levels of B-microglobulin and absolute CD4 counts. The expression of the co-stimulatory molecule CD28 was also greatly reduced on CD3+ lymphocytes from HIV-1+ patients due to the accumulation of both a CD8+CD57+ population that does not normally express CD28 and a novel CD8+CD57-CD28- population. The expression of CD28 correlated well with the number of blasts recovered after PHA activation (r = 0.66, p < 0.001). Only CD28+ cells transformed into blasts while CD28- cells transiently expressed IL-2Ra but did not undergo blastogenesis as measured by cell size and Brdu incorporation. Studies in which both CD28 and CD38 were investigated revealed that within the CD8 compartment a CD38+CD28- phenotype provided the best correlation with poor blast recovery.

*Acquired Immunodeficiency Syndrome/IMMUNOLOGY *Cell Death *HIV-1 *Lymphocyte Transformation *Lymphocytes/IMMUNOLOGY

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