translation agency

NLM AIDSLINE
Two cis-acting signals control ribosomal frameshift between human T-cell leukemia virus type II gag and pro genes.
Falk H; Mador N; Udi R; Panet A; Honigman A; Department of Molecular
December 30, 1993
J Virol. 1993 Oct;67(10):6273-7. Unique Identifier : AIDSLINE

The open reading frame of the human T-cell leukemia virus type II pro gene is arranged at a -1 position relative to the gag gene. Synthesis of the Gag-Pro fusion polyprotein is facilitated by ribosomal frameshift into the reading frame of the pro gene. Cloning of a synthetic 41-bp oligonucleotide corresponding to the gag-pro junction within a heterologous gene (nef of human immunodeficiency virus type I) and mutation analysis revealed that two cis-acting signals, an adenosine residue stretch and a dyad symmetry sequence, flanking the UAA termination codon, are required for efficient ribosomal frameshifting between gag and pro. The stability of the stem-loop structure is crucial for frameshifting.

Amino Acid Sequence Animal Base Sequence Cells, Cultured Cloning, Molecular DNA Mutational Analysis DNA, Viral/CHEMISTRY/*METABOLISM *Frameshift Mutation *Genes, gag Genes, nef *Genes, Viral Human HIV-1/GENETICS HTLV-II/*GENETICS Molecular Sequence Data Nucleic Acid Conformation *Open Reading Frames Ribosomes/*METABOLISM Support, Non-U.S. Gov't Terminator Regions (Genetics) Translation, Genetic Viral Proteins/BIOSYNTHESIS JOURNAL ARTICLE

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