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Enhanced expression of LFA-3 on human T-cell lines and leukemic cells carrying human T-cell-leukemia virus type 1.
Imai T; Tanaka Y; Fukudome K; Takagi S; Araki K; Yoshie O; Shionogi
March 30, 1994
Int J Cancer. 1993 Nov 11;55(5):811-6. Unique Identifier : AIDSLINE

Previously, we studied the surface expression of LFA-1 (CD11a/CD18) and ICAM-1 (CD54) in a panel of 16 human T-cell lines and found that those carrying HTLV-1 pro-viruses expressed high levels of ICAM-1. Enhanced expression of ICAM-1 was also seen in fresh leukemic cells from ATL patients. In the present study, we systematically examined the surface expression of 19 different cell-adhesion molecules (CAMs) (the immunoglobulin superfamily, the homing receptors, the integrins, etc.) in a panel of 20 human T-cell lines (6 HTLV-1-negative lines and 14HTLV-1-positive lines). The surface expression of LFA-3 (CD58) was constantly enhanced in HTLV-1-positive T-cell lines except for MT-1, an ATL-derived cell line, which was devoid of a number of CAMs including ICAM-1. LFA-3 proteins and mRNA were found at strongly increased levels in HTLV-1-positive T-cell lines.

Antigens, CD/*ANALYSIS/GENETICS Antigens, CD28/ANALYSIS Antigens, Differentiation, T-Lymphocyte/ANALYSIS Blotting, Northern Cell Adhesion Molecules/*ANALYSIS Flow Cytometry Fluorescent Antibody Technique Gene Expression Human HTLV-I/*IMMUNOLOGY Immunoblotting Integrins/ANALYSIS Leukemia, T-Cell/*IMMUNOLOGY Membrane Glycoproteins/*ANALYSIS/GENETICS Receptors, Immunologic/ANALYSIS Receptors, Lymphocyte Homing/ANALYSIS RNA, Messenger/ANALYSIS Sialoglycoproteins/ANALYSIS T-Lymphocytes/*IMMUNOLOGY JOURNAL ARTICLE