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Design and construction of rhinovirus chimeras incorporating immunogens from polio, influenza, and human immunodeficiency viruses.
Arnold GF; Resnick DA; Li Y; Zhang A; Smith AD; Geisler SC;
April 30, 1994
Virology. 1994 Feb;198(2):703-8. Unique Identifier : AIDSLINE

This paper describes the design and construction of chimeric human rhinoviruses that contain immunogenic regions from other pathogens as part of their surface coat proteins. Segments encoding the poliovirus 3 Sabin VP1 and VP2 proteins, the influenza hemagglutinin (HA) glycoprotein, and the human immunodeficiency virus gp120 surface and gp41 transmembrane glycoproteins were inserted into a full-length clone of human rhinovirus 14 (HRV14) at regions corresponding to neutralizing immunogenic sites IA (NIm-IA) and II (NIm-II). Of 12 chimeric constructs described, 3 produced viable virus. An HRV14 chimeric virus containing five amino acids of influenza HA (corresponding to 300 A2 of solvent-accessible surface area) had wild-type HRV14 growth characteristics and was neutralized by four of four anti-influenza HA antisera with reciprocal neutralizing titers ranging from 180 to 330. However, antisera raised in two guinea pigs against the HRV14:influenza HA chimera did not show significant neutralization of relevant strains of influenza. These results are the first to demonstrate the feasibility of making viable chimeras of human rhinoviruses displaying heterologous immunogens.

Amino Acid Sequence Antigens, Viral/*BIOSYNTHESIS/GENETICS/IMMUNOLOGY Capsid/BIOSYNTHESIS/GENETICS/*IMMUNOLOGY Chimeric Proteins/*BIOSYNTHESIS/GENETICS/IMMUNOLOGY Epitopes/*BIOSYNTHESIS/GENETICS/IMMUNOLOGY Hemagglutinins, Viral/GENETICS/IMMUNOLOGY HIV Envelope Protein gp120/GENETICS/IMMUNOLOGY HIV Envelope Protein gp41/GENETICS/IMMUNOLOGY Models, Molecular Molecular Sequence Data Polioviruses/GENETICS/IMMUNOLOGY Protein Engineering Rhinovirus/GROWTH & DEVELOPMENT/*GENETICS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE

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