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NLM AIDSLINE
Characterization and primary structure of a human immunodeficiency virus type 1 (HIV-1) neutralization domain as presented by a poliovirus type 1/HIV-1 chimera.
Vella C; Ferguson M; Dunn G; Meloen R; Langedijk H; Evans D; Minor PD;
April 30, 1994
J Gen Virol. 1993 Dec;74 ( Pt 12):2603-7. Unique Identifier : AIDSLINE

The poliovirus/human immunodeficiency virus (HIV) chimera S1/env/3 presents the sequence DRPEGIEEEGGERDRDRS, a known glycoprotein gp41 neutralizing domain (residues 735 to 752) of HIV IIIB in an antigenic site of the Sabin type 1 strain of poliovirus. Of 10 monoclonal antibodies raised against the sequence as presented in S1/env/3, eight were shown to neutralize HIV IIIB in vitro whereas all 10 neutralized S1/env/3, suggesting that the presentation of the sequence is comparable between HIV and the poliovirus/HIV chimera. The monoclonal antibodies were characterized by the selection of escape mutants from S1/env/3 and by Pepscan analysis. The two methods gave similar results, identifying two epitopes involving amino acids corresponding to residues 740 to 743, and to residues 745 to 750 of gp41. Mutations selected in the chimera with S1/env/3-specific MAbs are identical or similar to changes occurring in vivo in natural isolates of HIV-1. This finding suggests that the epitope may be significant in the neutralization of HIV in vivo.

Amino Acid Sequence Antibodies, Monoclonal/IMMUNOLOGY Antibody Specificity Binding Sites, Antibody/GENETICS/*IMMUNOLOGY Chimeric Proteins/IMMUNOLOGY Comparative Study Epitopes/GENETICS/*IMMUNOLOGY HIV Antibodies/GENETICS/*IMMUNOLOGY HIV Envelope Protein gp41/GENETICS/*IMMUNOLOGY HIV-1/GENETICS/*IMMUNOLOGY Molecular Sequence Data Mutagenesis Neutralization Tests Peptide Fragments/IMMUNOLOGY Polioviruses/GENETICS/IMMUNOLOGY Sequence Homology, Amino Acid Support, Non-U.S. Gov't JOURNAL ARTICLE

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