J Allergy Clin Immunol. 1994 May;93(5):831-5. Unique Identifier :
To determine the rate and completeness of delivery of venom from
honeybee stings, European bees were collected at the entrance of a hive
and studied with the use of two laboratory models. In one model bees
were induced to sting the shaved skin of anesthetized rabbits. The
stings were removed from the skin at various time intervals after
autotomization, and residual venom was assayed with a hemolytic method.
In the other model the bees were induced to sting preweighed filter
paper disks, which were weighed again after removal of the sting at
various intervals. Results of both experiments were in agreement,
showing that at least 90% of the venom sac contents were delivered
within 20 seconds and that venom delivery was complete within 1 minute.
The data suggest that a bee sting must be removed within a few seconds
after autotomization to prevent anaphylaxis in an allergic person. The
extensive variation found in the amount of venom delivered at each time
point may explain inconsistencies in relationships among reactions to
field stings, sting challenge testing, venom skin tests and RAST.
Anaphylaxis/PREVENTION & CONTROL Animal Bee Venoms/ADMINISTRATION &
DOSAGE/ANALYSIS/*PHARMACOKINETICS *Bees Comparative Study Insect
Bites and Stings/*PHYSIOPATHOLOGY Melitten/ANALYSIS Rabbits
Skin/PHYSIOPATHOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't,
P.H.S. Time Factors JOURNAL ARTICLE