translation agency

NLM AIDSLINE
Biological activity and intracellular location of the Tat protein of equine infectious anemia virus.
Rosin-Arbesfeld R; Mashiah P; Willbold D; Rosch P; Tronick SR; Yaniv A;
April 30, 1995
Gene. 1994 Dec 15;150(2):307-11. Unique Identifier : AIDSLINE

The Tat protein of equine infectious anemia virus (EIAV) was synthesized in Escherichia coli using the inducible expression plasmid, pET16b, which contains a His.Tag leader, thus allowing for rapid and efficient enrichment of the histidine-tagged protein by metal affinity chromatography. Yields of up to 20 mg of Tat were obtained from 10(11) bacterial cells. The recombinant Tat protein was shown to potently trans-activate the EIAV long terminal repeat (LTR) following its introduction into canine cells by 'scrape loading'. The EIAV Tat protein was found to localize predominantly within the cytoplasm, in contrast to HIV-1 Tat. The availability of large amounts of purified functional EIAV Tat protein should greatly facilitate detailed structure-function analyses.

Amino Acid Sequence Animal Base Sequence Cell Line Cloning, Molecular Comparative Study Dogs DNA Primers Escherichia coli Gene Products, tat/BIOSYNTHESIS/ISOLATION & PURIF/*METABOLISM HIV-1/METABOLISM Infectious Anemia Virus, Equine/GENETICS/*METABOLISM Molecular Sequence Data Plasmids Recombinant Fusion Proteins/METABOLISM Recombinant Proteins/BIOSYNTHESIS/ISOLATION & PURIF/METABOLISM *Repetitive Sequences, Nucleic Acid Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Templates *Trans-Activation (Genetics) JOURNAL ARTICLE

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