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T cell receptor repertoire of CD4+ and CD8+ T cell subsets in the allogeneic bone marrow transplant recipient.
Smith FS; Rencher SD; Heslop HE; Hurwitz JL; Department of Immunology,
December 30, 1995
Cancer Immunol Immunother. 1995 Aug;41(2):104-10. Unique Identifier :

Allogeneic bone marrow transplantation (BMT) has become a therapy of choice for the treatment of certain malignancies and hematopoietic disorders. However, immunodeficiencies following BMT continue to cause significant morbidity and mortality. We have compared the T cell receptor (TCR) repertoire of BMT patients and healthy control individuals by staining peripheral blood mononuclear cells with fluorochrome-labeled TCR-specific antibodies. Several patients exhibited a biased pattern of TCR expression atypical of the healthy controls, yet no particular TCR bias characterized all patients. For example, we found that 2%-8% of T cells from healthy individuals expressed the V beta 19 TCR. One BMT patient exhibited V beta 19 expression on more than 60% of peripheral T cells, while additional patients expressed V beta 19 on less than 1% of T cells. The patients with the most extreme skewing of TCR types suffered from graft-versus-host disease. The causes of skewed TCR V beta expression patterns in BMT patients are not fully understood, yet some researchers have suggested that an oligoclonal expansion of CD8+ T cell populations may be largely responsible. To test this hypothesis, we examined the TCR V beta repertoire of CD4+ and CD8+ T cell populations. We found that biased V beta expression characterized both CD4+ and CD8+ T cell populations, sometimes within a single individual. Thus, therapies directed toward CD8+ T cells alone may not fully correct repertoire abnormalities following BMT.

Adolescence Adult Antigens, CD3/*ANALYSIS Bone Marrow Transplantation/*IMMUNOLOGY Child Child, Preschool *CD4-CD8 Ratio CD4-Positive T-Lymphocytes/*CHEMISTRY CD8-Positive T-Lymphocytes/*CHEMISTRY Human Infant Receptors, Antigen, T-Cell, alpha-beta/*ANALYSIS Receptors, Antigen, T-Cell, gamma-delta/*ANALYSIS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Transplantation, Homologous JOURNAL ARTICLE

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