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Characterization of chronically infected cell lines and response to antiviral agents.
Halliday S; Buckheit R; Southern Research Institute-Frederick Research
December 30, 1995
Natl Conf Hum Retroviruses Relat Infect (1st). 1993 Dec 12-16;:133.

Chronically and latently infected cell lines have been derived following the acute infection of tissue culture cell lines with HIV-1. Although cell lines of this type are often used to investigate the role of inductive or inhibitory signals on HIV replication (U1, ACH-2, OM-10), little is known about the biological characteristics of these cell lines. We have biologically and biochemically evaluated chronically infected CEM, MT2, H9 and U937 cell lines to characterize the infected cells and compare them to the parental uninfected cells. In addition we have examined the derivation of this population from the acutely infected cells in terms of the virus infection. These studies were performed by single cell cloning of the parental population and virologic evaluation of the clones derived. Reverse transcriptase activity assays and PCR were performed to determine that chronically infected cells derived from cytopathically infected cell lines predominantly included uninfected cells with a small subpopulation of productively infected cells. In contrast, cell lines which were non- cytopathically infected yielded a majority of productively infected cells following acute infection. The clones derived from CEM and U937 cells responded differently to inductive signals such as TNF, IL6, and PMA than did either the U1 or ACH-2 cells. Finally, the activity of various antiviral agents was evaluated in our panel of chronically infected lines. Several agents were found to have an effect on the replication of HIV in these cultures. The mechanism of antiviral action of these compounds will be presented.

*Drug Resistance, Microbial Microbial Sensitivity Tests RNA-Directed DNA Polymerase/*ANTAGONISTS & INHIB ABSTRACT

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