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Molecular diagnosis of microsporidial opportunistic infections.
Pieniazek NJ; da Silva AJ; Slemenda SB; Visvesvara GS; Centers for
November 30, 1996
3rd Conf Retro and Opportun Infect. 1996 Jan 28-Feb 1;:160. Unique

Microsporidia are amitochondrial, obligate intracellular protozoan parasites infecting most taxonomic groups of animals. Recently, four species of microsporidia belonging to three genera ( Encephalitozoon,Enterocytozoon, and Septata) were repeatedly diagnosed as opportunistic infectious agents in immunodeficient patients. In humans, microsporidia cause intestinal, urinary tract, corneal, as well as disseminated infections. The most common diagnostic procedure for diagnosis of microsporidia infections is microscopic evaluation of stools, eye swabs, urine sediments, duodenal aspirates, as well as other diagnostic specimens. These diagnostic techniques are very inefficient have a low sensitivity, and in many cases do not give precise identification of the microsporidial species responsible for the infection. To improve clinical diagnosis of microsporidia, we developed species-specific Polymerase Chain Reaction (PCR) primers targeting the small subunit ribosomal RNA coding region. These primers were successfully used for sensitive, detection of Encephalitozoon cuniculi, E. hellem,Enterocytozoon bieneusi, and Septata intestinalis in biopsies, stools,urine sediments, and duodenal aspirates.

Human Microsporida/GENETICS/*ISOLATION & PURIF Polymerase Chain Reaction Protozoan Infections/*DIAGNOSIS/PARASITOLOGY ABSTRACT