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Variation in estimates of HIV-1 plasma RNA concentration among laboratories and assay kits.
Brambilla D; Yen-Lieberman B; Jackson B; Bremer J; Coombs R; Lin HJ;
November 30, 1996
3rd Conf Retro and Opportun Infect. 1996 Jan 28-Feb 1;:155. Unique

The AIDS Clinical Trials Group (ACTG) has undertaken a preliminary evaluation of the variation in estimates of plasma HIV-1 RNA among laboratories and among three commercially available kits for the quantitation of HIV-1 plasma RNA (Roche - Amplicor; Chiron - bDNA 1.0; Organon Teknika - NASBA) . Laboratories were sent a common set of standards and a coded panel of HIV-1 spiked serum samples for proficiency testing. To be proficient a laboratory had to be able to detect five-fold differences in RNA concentration. This analysis is based on data from laboratories that were proficient. We have tentatively concluded the following: 1. Inter-laboratory variation for laboratories using the same kits can be reduced using a common set of standards, although there is still some variation among laboratories. , and 2. Absolute RNA copy number differences among kits was consistent, although there was a small difference between the RNA concentration estimates for the Amplicor and NASBA kits.

HIV-1/GENETICS/*ISOLATION & PURIF Human RNA, Viral/*BLOOD Reagent Kits, Diagnostic/*STANDARDS Reference Standards Reproducibility of Results ABSTRACT