Int Conf AIDS. 1996 Jul 7-12;11(1):169 (abstract no. Mo.C.1668). Unique
Objective: To estimate the prevalence of HIV-1 and HIV-2 in Bombay and
investigate the interaction of HIV-1 and HIV-2 with respect to viral
replication in HIV-1/HIV-2 dually infected patients. Methods:Blood
samples were collected from 60 outpatients in G.T.Hospital, Bombay.
Seroprevalence of HIV-1 and HIV-2 were investigated by the particle
agglutination (PA) method and indirect immunofluorescence assay (IFA).
HIV-1 and HIV-2 proviral DNA in primary peripheral blood mononuclear
cells (PBMC) were detected by nested polymerase chain reaction (PCR).
Quantitative competitive polymerase chain reaction (QC-PCR) method was
used to quantify virion associated HIV-1 and HIV-2 RNA in plasma.
Results: According to the results of PA, IFA and PCR, 60 Bombay patients
were classified into five categories: 32 were HIV-1 single infection
(53.3%), 15 were HIV-1/HIV-2 dual infection (25%), 3 were HIV-2 single
infection (5%), 7 were HIV noninfection (11.7%) and 3 were indeterminate
(5%). Although the mean HIV-1 viral load of HIV-1/HIV-2 dually infected
group 79,330 copies/ml plasma) did not significantly different from that
of HIV-1 single infected group (86,640 copies/ml plasma), but we noted
the extremely separated distribution in HIV-1/HIV 2 dually infected
group ( less than 6,000 copies/ml plasma in 6 patients and greater than
160,000 copies/ml plasma in 4 patients), compared with distribution of
HIV-1 viral load in HIV-1 singly infected group. Despite the viral load
of HIV-2 lower than that of HIV-1 in the same HIV-1/HIV-2 dually
infected patients, we did not observe the significant difference of
HIV-2 viral load between the HIV-1/HIV-2 dually infected group and HIV-2
single infected group.(This comparison may lack statistical power due to
small number of HIV-2 single infected patients.)Conclusion: In Bombay,
HIV-1 infection is predominant and HIV-1/HIV-2 dual infection is more
common than HIV-2 single infection. In some dually infected patients,
the existence of HIV-2 may have some inhibitory effect, on HIV-1
replication, but the order of HIV infection and subtype of HIV-2 may be
crucial: whether HIV-1 can not replicate well in previously HIV-2
infected patients or whether only some subtype of HIV-2 inhibits HIV-1
replication require further study.
*HIV Infections/EPIDEMIOLOGY *HIV-1/ISOLATION & PURIF *HIV-2/ISOLATION