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[Structural and functional analysis of the human immunodeficiency glycoprotein gp120 with homologous synthetic peptides]
Ketlinskii SA; Kalinina NM; Prusakov AN; Kaurov OA
February 28, 1997
Vestn Ross Akad Med Nauk. 1996;(8):9-12. Unique Identifier : AIDSLINE

The purpose of the study was to identify sites of gp 120, which are responsible for CD4 binding and induce tumor necrosis factor-alpha (TNF-alpha) synthesis. Seven peptides were synthesized from gp 120. The peptides were studied in the following biological tests: binding to CD4 molecules of the Jurkat cell clones 3G6 and PBMC of healthy persons. There was TNF-alpha induction in healthy and HIV-positive individuals and its correlation was found with p24 antigenemia in HIV patients. The peptides 420-440, 426-452, 369-384, 255-272 bind to T-lymphocytic CD4 and induced TNF-alpha. The peptide 436-451 binds to CD4, but failed to induced TNF-alpha, which suggests that the latter may be used as a basis for HIV-infection vaccine.

Antigens, CD4/METABOLISM English Abstract Human HIV Envelope Protein gp120/*CHEMISTRY/*PHYSIOLOGY HIV Infections/METABOLISM *HIV-1 Protein Binding Tumor Necrosis Factor/METABOLISM JOURNAL ARTICLE