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Direct cytotoxicity of HIV-1 envelope protein gp120 on human NT neurons.
Wu P; Price P; Du B; Hatch WC; Terwilliger EF; Division of
February 28, 1997
Neuroreport. 1996 Apr 10;7(5):1045-9. Unique Identifier : AIDSLINE

A new in vitro system comprising a pure population of neurons, human NT cells, was used to characterize the direct neurotoxic effect of HIV-1 envelope protein gp120. Cytotoxicity was monitored by a quantitative assay after exposure to recombinant gp120 in the presence or absence of other reagents. Treatment of mature NT neurons with various doses of gp120 for 24 h caused a decrease of up to 27% in the number of viable cells. This neurotoxicity was abolished by co-treatment with either D-2-amino-5-phosphonopentanoic acid (APV), MK801 or nimodipine, or by culturing cells in a Ca(2+)-free environment. Taken together, these data indicate that gp120 exerts a direct neurotoxic effect by acting through NMDA receptors and Ca2+ channels.

Dizocilpine Maleate/PHARMACOLOGY Excitatory Amino Acid Agonists/PHARMACOLOGY Human HIV Envelope Protein gp120/*TOXICITY *HIV-1 Neurons/*DRUG EFFECTS Neuroprotective Agents/PHARMACOLOGY Receptors, N-Methyl-D-Aspartate/AGONISTS Recombinant Proteins/TOXICITY Support, U.S. Gov't, P.H.S. Tumor Cells, Cultured 2-Amino-5-phosphonovalerate/PHARMACOLOGY JOURNAL ARTICLE