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A simplified semiquantitative determination of hepatitis C virus genome molecules by the end-point dilution method.
Bathelier C; Mercier G; Lucotte G; LCL Laboratory, Paris, France.
June 30, 1997
Mol Cell Probes. 1996 Dec;10(6):477-80. Unique Identifier : AIDSLINE

We describe a semiquantitative method to measure hepatitis C virus (HCV) viral particle numbers, by carrying out reverse-transcription polymerase chain reaction (RT-PCR) on serial dilutions of serum samples. The virus concentrations measured were 10(3)-10(6) viral particles ml-1 of serum. The method described is relatively quick, and the only required manipulation is dilution of the serum. An optimal RT-PCR method is used for diluted and undiluted samples.

*Hepatitis C/VIROLOGY *Hepatitis C-Like Viruses/ISOLATION & PURIF *RNA, Viral/BLOOD *Viral Load/METHODS