J Med Vet Mycol. 1996 Nov-Dec;34(6):371-8. Unique Identifier : AIDSLINE
Penicillium marneffei is one of the most important opportunistic
infectious pathogens in AIDS patients in Thailand and Southeast Asia.
However, very little is known about the host defence mechanisms against
P. marneffei infection. In the present study, we established the first
experimental murine model of chronic pulmonary and disseminated
infection using P. marneffei, and examined the immunological response to
such infection in euthymic and athymic mice. In this model,
micro-organisms inoculated intratracheally multiplied progressively in
the lungs and disseminated to the liver and spleen. However, the number
of organisms decreased gradually in these organs. In contrast,
congenitally athymic mice developed severe pulmonary and disseminated
systemic mycosis. Pulmonary penicilliosis marneffei was associated with
a marked cellular inflammatory response as evident by histological
abnormalities and increased intraparenchymal leucocyte count. To confirm
the importance of cell-mediated immunity in host resistance to P.
marneffei infection, we transferred nylon wool non-adherent spleen cells
into the athymic mice. Such treatment significantly reduced the number
of yeasts in the organs of athymic mice. Taken together, our results
demonstrate that the cell-mediated immunity play a central role in a
host defence mechanism against infection with P. marneffei, and suggest
that our new model may be a useful approach for studying the
pathogenesis of this fungal disease.
*Mycoses/IMMUNOLOGY *Penicillium/IMMUNOLOGY *T-Lymphocytes/IMMUNOLOGY