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Human herpes virus-8 DNA in bronchoalveolar lavage samples from patients with AIDS-associated pulmonary Kaposi's sarcoma.
Benfield TL; Dodt KK; Lundgren JD; Department of Infectious Diseases,
September 30, 1997
Scand J Infect Dis. 1997;29(1):13-6. Unique Identifier : AIDSLINE

Kaposi's sarcoma (KS) is the most frequent AIDS-associated neoplasm, and often disseminates to visceral organs, including the lungs. An ante-mortem diagnosis of pulmonary KS is difficult. Recently, DNA sequences resembling a new human herpes virus HHV-8), have been identified in various forms of KS. We hypothesized that these sequences are present in samples obtained by bronchoalveolar lavage (BAL) in patients with pulmonary KS. Utilizing a nested polymerase chain reaction (PCR), 7/12 BAL cell samples from HIV-infected patients with endobronchial KS were positive for HHV-8 DNA. In contrast, only 2/39 samples from HIV-infected patients without evidence of KS were positive (p = 0.007). Detection of HHV-8 in BAL cells of patients with pulmonary KS was highly specific (95%), with a sensitivity of 58% and a positive predictive value of 78%. In conclusion, HHV-8 is associated with pulmonary KS, and PCR amplification of HHV-8 in BAL cells provides a non-invasive method with a high positive predictive value.

*AIDS-Related Opportunistic Infections/VIROLOGY *Bronchoalveolar Lavage Fluid/VIROLOGY *DNA, Viral/ANALYSIS *Herpesvirus, Kaposi's Sarcoma-Associated/ISOLATION & PURIF *Lung Neoplasms/VIROLOGY *Sarcoma, Kaposi's/VIROLOGY

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