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Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy.
Chun TW; Stuyver L; Mizell SB; Ehler LA; Mican JA; Baseler M; Lloyd AL;
March 30, 1998
Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13193-7. Unique Identifier

Although highly active antiretroviral therapy (HAART) in the form of triple combinations of drugs including protease inhibitors can reduce the plasma viral load of some HIV-1-infected individuals to undetectable levels, it is unclear what the effects of these regimens are on latently infected CD4+ T cells and what role these cells play in the persistence of HIV-1 infection in individuals receiving such treatment. The present study demonstrates that highly purified CD4+ T cells from 13 of 13 patients receiving HAART with an average treatment time of 10 months and with undetectable (<500 copies HIV RNA/ml) plasma viremia by a commonly used bDNA assay carried integrated proviral DNA and were capable of producing infectious virus upon cellular activation in vitro. Phenotypic analysis of HIV-1 produced by activation of latently infected CD4+ T cells revealed the presence in some patients of syncytium-inducing virus. In addition, the presence of unintegrated HIV-1 DNA in infected resting CD4+ T cells from patients receiving HAART, even those with undetectable plasma viremia, suggests persistent active virus replication in vivo.

*Anti-HIV Agents/THERAPEUTIC USE *HIV Infections/VIROLOGY *HIV-1/PHYSIOLOGY *Virus Latency

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