translation agency

NLM AIDSLINE
A prospective study of HHV8 prevalence in patients presenting at a STD department.
Masquelier B; Cazorla C; Simon A; Caminade P; Geniaux M; Dupon M; Fleury
December 30, 1998
Int Conf AIDS. 1998;12:1095-6 (abstract no. 60519). Unique Identifier :

BACKGROUND: Kaposi's Sarcoma-associated herpesvirus (HHV8), has been shown to be mainly restricted to some populations, i.e. HIV-infected homosexuals, in the western developped countries. In order to study the prevalence of HHV8 infection in different populations in France, we set up a prospective study in a STD department. METHODS: During one year, persons presenting at the Bordeaux Hospital STD clinic for anonymous HIV screening were proposed to be enrolled in the study which corresponded to a blood sampling and a questionnaire regarding the sexual behaviour. HHV8 DNA extracted from the PBMC was submitted to a 38-cycle PCR followed by liquid hybridization and ELISA detection, blindy in regard with the caracteristics of the patients. Confirmation of positive results using non overlapping primers and serological studies are in progress. RESULTS: Between April 1996 and October 1997, 153 persons entered into our study. Two different groups were enrolled: a group of 102 heterosexuals, with a sex ratio at 1.7, and a group of 51 homo- or bisexual men. Results are expressed in table: TABULAR DATA, SEE ABSTRACT VOLUME. Noteworthy, the HHV8 DNA prevalence was 7.3% in the group of heterosexual men, and in contrast 0% for the women. CONCLUSION: HHV8 prevalence, measured by PCR, exhibited a positive gradient from female population to male heterosexual patients, and to homo- or bisexual men, in persons presenting at a STD clinic. These findings may support the hypothesis of the importance of sexual behaviour in regard with HHV8 transmission.

MEETING ABSTRACTS Female France/EPIDEMIOLOGY Herpesviridae Infections/*EPIDEMIOLOGY Herpesvirus, Kaposi Sarcoma-Associated/*ISOLATION & PURIF Human Male Prevalence Prospective Studies Sex Behavior Sexually Transmitted Diseases/*EPIDEMIOLOGY/*VIROLOGY Tumor Virus Infections/*EPIDEMIOLOGY

www.aegis.org