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Differential regulation of Th1 and Th2 cells by p91-110 and p21-40 peptides of the 16-kD alpha-crystallin antigen of Mycobacterium tuberculosis.
Agrewala JN; Wilkinson RJ; Tuberculosis & Related Infections Unit, MRC
March 30, 1999
Clin Exp Immunol. 1998 Dec;114(3):392-7. Unique Identifier : AIDSLINE

Permissively recognized peptides which can activate lymphocytes from subjects with a variety of class II HLA types are interesting diagnostic and vaccine candidates. In this study we generated T helper clones reactive to the permissively recognized p21-40 and p91-110 peptides of the 16-kD heat shock protein of Mycobacterium tuberculosis. All the clones specific for p91-110 secreted interferon-gamma (IFN-gamma) and were of the Th1 phenotype. By contrast, the p21-40 peptide favoured the generation of IL-4-producing clones. Antibody blockade established that the peptide-specific Th clones could either be DR-, DP- or DQ-restricted. Thus, two permissively recognized sequences p21-40 and p91-110 from the same mycobacterial antigen can drive the differentiation of functionally distinct T helper subsets. Attempts to immunize against tuberculosis should bear in mind epitope specificity if a favourable Th subtype response is to be generated.

JOURNAL ARTICLE Amino Acid Sequence Antigens, Bacterial/*IMMUNOLOGY Cell Line Cytokines Dose-Response Relationship, Drug Human HLA-DP Antigens/IMMUNOLOGY HLA-DQ Antigens/IMMUNOLOGY HLA-DR Antigens/IMMUNOLOGY Immunophenotyping Molecular Sequence Data Mycobacterium tuberculosis/*IMMUNOLOGY Peptide Fragments/IMMUNOLOGY Th1 Cells/*IMMUNOLOGY Th2 Cells/*IMMUNOLOGY