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Molecular cloning and phylogenetic analysis of human immunodeficiency virus type 1 subtype C: a set of 23 full-length clones from Botswana.
Novitsky VA; Montano MA; McLane MF; Renjifo B; Vannberg F; Foley BT;
July 30, 1999
J Virol. 1999 May;73(5):4427-32. Unique Identifier : AIDSLINE

To better understand the virological aspect of the expanding AIDS epidemic in southern Africa, a set of 23 near-full-length clones of human immunodeficiency virus type 1 (HIV-1) representing eight AIDS patients from Botswana were sequenced and analyzed phylogenetically. All study viruses from Botswana belonged to HIV-1 subtype C. The interpatient diversity of the clones from Botswana was higher than among full-length isolates of subtype B or among a set of full-length HIV-1 genomes of subtype C from India (mean value of 9. 1% versus 6.5 and 4.3%, respectively; P < 0.0001 for both comparisons). Similar results were observed in all genes across the entire viral genome. We suggest that the high level of HIV-1 diversity might be a typical feature of the subtype C epidemic in southern Africa. The reason or reasons for this diversity are unclear, but may include an altered replication efficiency of HIV-1 subtype C and/or the multiple introduction of different subtype C viruses.

JOURNAL ARTICLE Base Sequence Botswana/EPIDEMIOLOGY Cloning, Molecular DNA, Viral Human HIV Infections/EPIDEMIOLOGY/*VIROLOGY HIV-1/CLASSIFICATION/*GENETICS Molecular Sequence Data Phylogeny Support, U.S. Gov't, P.H.S.