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Cutting edge: activation of NK T cells by CD1d and alpha-galactosylceramide directs conventional T cells to the acquisition of a Th2 phenotype.
Singh N; Hong S; Scherer DC; Serizawa I; Burdin N; Kronenberg M; Koezuka
November 30, 1999
J Immunol. 1999 Sep 1;163(5):2373-7. Unique Identifier : AIDSLINE

NK T cells recognize glycolipid Ags such as alpha-galactosylceramide (alpha-GalCer) presented by the MHC class I-like molecule CD1d. In this paper we have studied the in vivo effects of alpha-GalCer on the generation of adaptive immune responses. Treatment of mice with alpha-GalCer resulted in rapid activation of NK T cells and production of the cytokines IL-4 and IFN-gamma. However, after this initial stimulation, NK T cells became polarized for the production of IL-4. Further, as soon as 6 days after alpha-GalCer injection, a marked increase in serum IgE levels was observed. Administration of alpha-GalCer at the time of priming of mice with protein Ag resulted in the generation of Ag-specific Th2 cells and a profound increase in the production of IgE. Collectively, these findings indicate that alpha-GalCer may be useful for modulating immune responses toward a Th2 phenotype during prophylaxis and therapy.

JOURNAL ARTICLE Adjuvants, Immunologic/ADMINISTRATION & DOSAGE Animal Antigens, CD1/GENETICS/*IMMUNOLOGY B-Lymphocytes/IMMUNOLOGY Cell Differentiation/IMMUNOLOGY Cytokines/BIOSYNTHESIS Galactosylceramides/ADMINISTRATION & DOSAGE/*IMMUNOLOGY Immunity, Cellular/IMMUNOLOGY Immunophenotyping Injections, Intraperitoneal Killer Cells, Natural/*IMMUNOLOGY/METABOLISM Lymphocyte Transformation/*IMMUNOLOGY Mice Mice, Inbred C57BL Mice, Mutant Strains T-Lymphocyte Subsets/*IMMUNOLOGY/METABOLISM Th1 Cells/METABOLISM Th2 Cells/IMMUNOLOGY/*METABOLISM