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Compartmentalization of the IgG immune response to HIV-1 in breast milk.
Becquart P; Hocini H; Garin B; Sepou A; Kazatchkine MD; Belec L; Unite
December 30, 1999
AIDS. 1999 Jul 30;13(11):1323-31. Unique Identifier : AIDSLINE

OBJECTIVE: To evaluate the IgG immune response to HIV-1 in colostrum. METHODS: Paired serum and colostrum were collected from 16 asymptomatic HIV-1-infected women. IgG to gp160 and to four peptides (gp41 immunodominant DI domain, gp41/Id; EDLKWA epitope of DIII domain, gp41/K; gp120 C-terminus, gp120/Ct; V3 loop, gp120/V3) were evaluated in all samples. Functional activity of purified IgG was assessed for the ability to block transcytosis of cell-associated HIV-1 through a tight monolayer of endometrial epithelial cell line HEC1. RESULTS: IgG antibody to gp160 and to the four env-encoded synthetic peptides were detected in all specimens. The mean specific activity of IgG to gp41/K was 4.2 fold higher in colostrum than in paired serum. In contrast, mean specific activities of IgG to gp160 and gp41/Id were twofold higher in serum than in paired colostrum. Mean specific activities of IgG to gp120/V3 and to gp120/Ct were similar in systemic and milk compartments. Functional activity of IgG was evaluated in six paired serum and colostrum: in two women, serum IgG was 3.0 and 7.6 fold more efficient in blocking transcytosis than colostrum IgG; in one patient, colostrum IgG exhibited a 28 fold higher inhibitory capacity than serum IgG; in the remaining patients, serum and colostrum IgG demonstrated similar inhibitory activities against transcytosis of HIV. CONCLUSION: These features are consistent with a compartmentalization of the humoral IgG immune response to HIV within the mammary gland. Some HIV-1 antigens are able to induce a strong humoral mucosal immune response which may be of relevance for the design of a mucosal vaccine against HIV-1.

JOURNAL ARTICLE Adolescence Adult Animal Breast Feeding Cells, Cultured Colostrum/IMMUNOLOGY Endocytosis/DRUG EFFECTS Epitopes Female Human HIV Antibodies/*ANALYSIS/BLOOD HIV Antigens/IMMUNOLOGY HIV Envelope Protein gp120/CHEMISTRY/IMMUNOLOGY HIV Envelope Protein gp160/IMMUNOLOGY HIV Envelope Protein gp41/IMMUNOLOGY HIV Infections/*IMMUNOLOGY HIV-1/*IMMUNOLOGY/PHYSIOLOGY IgG/*ANALYSIS/BLOOD/IMMUNOLOGY Immunity, Mucosal Milk, Human/*IMMUNOLOGY Peptide Fragments/CHEMISTRY/IMMUNOLOGY Pregnancy Pregnancy Complications, Infectious/*IMMUNOLOGY/VIROLOGY Support, Non-U.S. Gov't