translation agency

NLM AIDSLINE
Inhibition of infection of incoming HIV-1 virus by RNA-cleaving DNA enzyme.
Zhang X; Xu Y; Ling H; Hattori T; Division of Allergy and Infectious
December 30, 1999
FEBS Lett. 1999 Sep 17;458(2):151-6. Unique Identifier : AIDSLINE

Nine different DNA enzymes (DzV3-n, n=1-9) targeting the V3 loop region of HIV-1 HXB2 were synthesized. One of those, DzV3-9, efficiently cleaved the target in the conserved sequence in the RNA transcript in vitro. DzV3-9 was stable in the cells and inhibited replication of both NL432 and SF162 strains in U87 cells expressing CD4 and co-receptors. The inhibitory effect of DNAzyme on incoming HIV-1 was also demonstrated with pseudotype virions generated by NL432-based luciferase reporter genes. Thus, an efficient, stable DNAzyme against a functionally important region of HIV-1 was identified, and it may be useful for prevention of HIV-1 infection.

JOURNAL ARTICLE Antiviral Agents/CHEMICAL SYNTHESIS/*PHARMACOLOGY Base Sequence Catalysis Cell Line DNA, Single-Stranded/CHEMICAL SYNTHESIS/*PHARMACOLOGY DNA, Viral/CHEMICAL SYNTHESIS/*PHARMACOLOGY Enzyme Stability Gene Products, env/METABOLISM Human Hydrolysis HIV Infections/ENZYMOLOGY/PREVENTION & CONTROL/VIROLOGY HIV-1/*DRUG EFFECTS/PHYSIOLOGY/PATHOGENICITY Kinetics Molecular Sequence Data Peptide Fragments/METABOLISM RNA, Viral/*METABOLISM Support, Non-U.S. Gov't Virus Replication

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