Panminerva Med. 1999 Jun;41(2):93-7. Unique Identifier : AIDSLINE
BACKGROUND: Many data suggest T cell functional impairment in B-cell
chronic lymphocytic leukemia (B-CLL). The mechanism responsible for this
phenomenon is still unresolved. METHODS: In 88 B-CLL patients (RAI
II-IV) the relationship between immunoregulatory T cells and PHA induced
lymphoproliferative response (LPR) was analysed before and after the
therapy. The number of peripheral blood CD3+, CD4+ and CD8+ T
lymphocytes was determined by indirect immunofluorescence assay using
monoclonal antibodies. LPR was estimated in whole blood culture method.
RESULTS: The absolute number of CD3+, CD4+ and CD8+ cells in untreated
CLL patients was much higher than in healthy controls (n = 26), but the
percentages of these subpopulations, CD4/CD8 ratio and LPR to PHA were
significantly (p < 0.00001) decreased. The chemotherapy induced a
significant rise of CD3+ and CD4+ percentages (p < 0.006 < p < 0.022
respectively) in comparison to baseline levels, but their levels
remained significantly (p < 0.00001) lower than the controls. The
CD4/CD8 ratio was also elevated after the therapy (p < 0.048) but
remained below the normal value as well. The absolute number of CD3+ and
CD4+ T cells were normalized after treatment, while the CD8+ cells were
still higher (p < 0.044) than controls. The increase of LPR has been
registered after treatment, but it failed to reach the control values.
We could not find any correlation between the number of immunoregulatory
T cells and induced LPR (r = 0.07, for CD4+; r = 0.09 for CD8+ cells).
CONCLUSIONS: These data indicate some profound lymphoid cell defect in
CLL patients affecting CD8+ proliferation as well as LPR.
JOURNAL ARTICLE Adolescence Adult CD4-CD8 Ratio/DRUG EFFECTS Female
Human Leukemia, B-Cell, Chronic/DRUG THERAPY/*IMMUNOLOGY/PATHOLOGY
Lymphocyte Count/DRUG EFFECTS *Lymphocyte Transformation/DRUG
EFFECTS/IMMUNOLOGY Male Middle Age