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9th Annual Conference Of The British HIV Association [BHIVA]24 – 26 April 2003, University of Manchester
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[AUTHOR(S):] S Portsmouth, J Stebbing, MR Nelson, S Patterson, FM Gotch and BG Gazzard
Chelsea and Westminster Hospital, London, UK
BHIVA Conf 2003 Apr 24-26;9:O15
OBJECTIVES: There is widespread recognition of the potential morbidity and mortality associated with HIV and hepatitis C (HCV) co-infection. To assess the feasibility of using autologous dendritic cells (DCs) in immunotherapy and to further elucidate this interaction, we examined the capacity of peripheral blood DCs recovered from co-infected individuals to stimulate naïve T cells.
METHODS: Monocyte-derived DCs were generated from HIV-1 seropositive patients with (n=10) and without (n=10) HCV infection. These individuals were matched for HIV-1 virological and immunological parameters. We performed mixed leucocyte reactions with cell division analyses, using carboxy fluorosuccinyl ester (CSFE), a fluorinated vital dye, in order to examine the allostimulatory potentials of DCs.
RESULTS: Monocyte-derived DCs from co-infected individuals showed a similar allostimulatory capacity to in vitro generated DCs from HIV-1 infected individuals without HCV. This impairment was not reversed by increasing concentrations of either exogenous interleukin-2 or –12.
CONCLUSIONS: Monocyte-derived DCs from HIV-1 and HCV co-infected individuals have a similar allostimulatory capacity to DCs from patients with HIV-1. This has implications for immunotherapeutic approaches in co-infected individuals and is consistent with recent data showing that HCV and HIV-1 do not negatively affect one another.
PRESENTING AUTHOR: S Portsmouth
030424
O15
Copyright © 2003 - British HIV Association (BHIVA) Reproduction of this abstract (other than one copy for personal reference) must be cleared through the BHIVA Organising Secretariat 1 Mountview Court, 310 Friern Barnet Lane, London N20 0LD