AEGiS-05CROI: Abnormal T cell receptor repertoire in children with vertically-acquired HIV infection.

5th Conference on Retroviruses and Opportunistic Infections


Chicago, IL - February 1-5, 1998



Abnormal T cell receptor repertoire in children with vertically-acquired HIV infection.

Conf Retroviruses Opportunistic Infect 1998 Feb 1-5; 5th:82 (abstract no. 18)

McFarland EJ, Harding PA, Striebich CC, Kuritzkes DR, Kotzin BL; University of Colorado Health Science Center, Denver, CO.

We studied the T cell receptor (TCR) repertoire in 24 infants and children with vertically-acquired HIV infection. PBMC were analyzed by immunofluorescence staining and cytofluorographic analysis using a panel of anti-TCR mAb covering approximately 50% of the Vbeta repertoire. Expansions were defined based on data from cord blood and samples from 9-month old infants. Whereas, only 2.6% of healthy, 4-12yr old children had CD8 subset expansions, 67% of the HIV-infected subjects had CD8 T cell expansions occupying up to 25% of the entire CD8 repertoire. Sequence analysis of the TCR junctional region revealed a predominant clone in most cases. The frequency of the clones in the total CD8 cells ranged from 4-11% and the absolute number in peripheral blood ranged from 38-157 cells/ml. Each expanded CD8Vbeta subset also contained 2-6 smaller clonal expansions. The expansions were primarily in the CD8+CD28- fraction, but were often present in the CD8+CD28+ population as well. Sequence analysis of sorted cells confirmed that the expanded clones were present in both the CD28+ and CD28- fractions. HIV-specific stimulation of PBMC induced HIV-specific cytotoxicity, and, in several cases resulted in a 3-8 fold increase in the relative proportion of cells bearing the Vbeta expressed by the expanded subset. Sequence analysis confirmed an increase in the number of cells with identical TCR junctional sequences following stimulation. This data demonstrates that HIV-infected children have expanded CD8 T cell clones occupying a large proportion of their T cell repertoire.

Keywords: AEGIS, Receptors, Antigen, T-Cell, HIV Infections, Antigens, CD28, Antigens, CD8, HIV, T-Lymphocytes, Clone Cells, HIV Antigens, Child, Human, Infant, AIDSKWDaegis,receptors,antigen,t-cell,hivinfections,antigens,cd28,antigens,cd8,hiv,t-lymphocytes,clonecells,hivantigens,child,human,infant,aids

1998-02-01
18

Copyright © 1998 - Foundation for Retrovirology and Human Health (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the Foundation for Retrovirology and Human Health. Licensed from National Library of Medicine.