AEGiS-05CROI: M-tropic HIV-1 strains are extremely sensitive to inhibition by RANTES. Sensitivity of T-tropic strains to RANTES is donor cell dependent.

5th Conference on Retroviruses and Opportunistic Infections


Chicago, IL - February 1-5, 1998



M-tropic HIV-1 strains are extremely sensitive to inhibition by RANTES. Sensitivity of T-tropic strains to RANTES is donor cell dependent.

Conf Retroviruses Opportunistic Infect 1998 Feb 1-5; 5th:83 (abstract no. 23)

Trkola A, Vesanen M, Monard S, Siani MA, Hoxie JA, Wu L, Moore JP; Aaron Diamond AIDS Research Center, Rockefeller University, New York, NY.

We have studied the breadth and potency of the inhibitory action of CC-chemokines MIP-1a, MIP-1beta and RANTES against M-tropic strains from subtypes A through F and found that RANTES is by far the most potent of these chemokines in inhibiting virus replication in CD4+ T-cells. This inhibition is subtype independent but a high inter-isolate variation was noticed. The influence of RANTES on replication of T-tropic isolates in CD4+T-cells shows diverse, donor cell dependent patterns. In some donor cells (including cells homozygous for a deletion in CCR5) treatment with high concentrations of RANTES before infection resulted in inhibition of virus replication, whereas in other donor cells enhancement of replication was observed. None of the other two tested beta-chemokines had inhibitory effects on T-tropic isolates. To characterize the inhibitory effect of RANTES in more detail we monitored changes in levels of expression of co-receptors and adhesion molecules in response to treatment with the chemokine. We found that RANTES down-regulates CCR5 expression more potently than MIP-1alpha and MIP-1beta and also affects the expression of several adhesion molecules on the surface of activated CD4+T-cells. Changes in adhesion molecule expression upon RANTES treatment is also observed on cells homozygous for a deletion in CCR5 indicating that a chemokine receptor other than CCR5 is involved. The observed changes in the expression of adhesion molecules and co-receptors might contribute to the anti-viral activity of RANTES and also could account for its greater potency.

Keywords: AEGIS, RANTES, HIV-1, Macrophage Inflammatory Protein-1, Virus Replication, Chemokines, CC, Anti-HIV Agents, Chemokines, T-Lymphocytes, Antigens, CD4, virology, AIDSKWDaegis,rantes,hiv-1,macrophageinflammatoryprotein-1,virusreplication,chemokines,cc,anti-hivagents,chemokines,t-lymphocytes,antigens,cd4,virology,aids

1998-02-01
23

Copyright © 1998 - Foundation for Retrovirology and Human Health (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the Foundation for Retrovirology and Human Health. Licensed from National Library of Medicine.