AEGiS-05CROI: Co-operative interaction of V(1)-V(2) region with V(3) region of HIV-1 gp120 is important for productive viral infection: its role in binding of gp120 to viral co-receptor.

5th Conference on Retroviruses and Opportunistic Infections


Chicago, IL - February 1-5, 1998



Co-operative interaction of V(1)-V(2) region with V(3) region of HIV-1 gp120 is important for productive viral infection: its role in binding of gp120 to viral co-receptor.

Conf Retroviruses Opportunistic Infect 1998 Feb 1-5; 5th:84 (abstract no. 28)

Chen M, Gupta P; University of Pittsburgh, PA.

The env gene of HIV-1, particularly the V(3) region of gp120, has been implicated in replication, cell tropism and in vitro cytopathogenicity. However, there is little information on the interaction between the V(3) region and other hypervariable regions of gp120, and its effect on these biologic properties. To determine such interactions, recombinant viruses were constructed between two infectious molecular clones of HIV-1, ME1 and ME46. ME1 had a low replicating, macrophage tropic and non-syncytia inducing property, while clone ME46 had high a replicating, dual tropic and syncytia inducing property. Examination of the biologic properties of the recombinants showed that although high replication and cytopathogenicity are controlled by the env region of ME46, replacement of V(1)-V(2) region of ME46 with the corresponding region of ME1 rendered the virus totally replication incompetent. Such negative interaction between V(1)-V(2) and V(3) regions of viral replication was independent on whether the 5' half of the genome (5' LTR-Vpr) was derived from ME1 or ME46. Analysis of the env sequence of ME1 and ME46 shows two large deletions in the V(1) region. These results indicate that there is a cooperative interaction between the V(3) and V(1)-V(2) regions that is important for viral replication. It is possible that such deletions in the V(1) region could block conformational changes in the V(3) region that are required for the interaction of gp120 with viral coreceptors (CCR5, CXCR4).

Keywords: AEGIS, HIV-1, Virus Replication, Genes, env, Drug Interactions, HIV-1 Reverse Transcriptase, Macrophages, Carrier Proteins, Cobalt, In Vitro, virology, pharmacokinetics, genetics, AIDSKWDaegis,hiv-1,virusreplication,genes,env,druginteractions,hiv-1reversetranscriptase,macrophages,carrierproteins,cobalt,invitro,virology,pharmacokinetics,genetics,aids

1998-02-01
28

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