5th Conference on Retroviruses and Opportunistic Infections Chicago, IL - February 1-5, 1998

Conf Retroviruses Opportunistic Infect 1998 Feb 1-5; 5th:85 (abstract no. 33)

Bastiani L, Laal S, Hoxie JA, Zolla-Pazner S, Bandres JC; New York University Medical Center, New York, NY.

Both primary isolates and laboratory strains of HIV-1 incorporate different cell-derived molecules into their envelopes depending on the host cell in which they are grown. This incorporation in not random; for example, HIV-1 has been shown to exclude CD4 from its envelope. In this study we have examined the incorporation into the envelope of HIV-1 of three coreceptors -- CXCR4,CCR5, and CCR3-- as well as CD4. For this purpose, the HIV-1 SI primary isolate BZ167 was passaged into PHA-stimulated PBMC and CEM-SS cells and the incorporation of various cell-derived molecules into the virion envelope was determined with a virus binding ELISA. As previously shown, BZ167 grown in PHA-stimulated PBMC expressed most of the adhesion molecules tested (such as LFA-1, ICAM-1, and MHC class II), while CEM-SS-grown BZ167 did not express significant levels of any of these adhesion molecules. CEM-SS cells express both CD4 and CXCR4 whereas PHA-stimulated PBMCs express CD4, CXCR4, CCR5, and CCR3. In contrast, BZ167 grown in either cell type lacked CD4 as well as the coreceptors, CXCR4, CCR5, and CCR3.

1998-02-01
33

Copyright © 1998 - Foundation for Retrovirology and Human Health (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the Foundation for Retrovirology and Human Health. Licensed from National Library of Medicine.