AEGiS-06CROI: Antigenicity and immunogenicity of HIV-1 envelope glycoproteins derived either from primary isolates or T-cell line adapted strains.

6th Conference on Retroviruses and Opportunistic Infections


Chicago, IL - January 31-February 4, 1999



Antigenicity and immunogenicity of HIV-1 envelope glycoproteins derived either from primary isolates or T-cell line adapted strains.

Conf Retroviruses Opportunistic Infect 1999 Jan 31-Feb 4; 6th:76 (abstract no. 48)

Brand D, Lemiale F, Thibault G, Verrier B, Buzelay L, Brunet S, Barin F; University F Rabelais, Tours, France.

Objective: to study the antigenic and immunogenic properties of HIV-1 envelope glycoproteins derived from 3 primary isolates and 2 T-cell line adapted strains (TCLA) expressed using the Semliki Forest Virus (SFV) system.

Methods: the env genes from 2 TCLA strains (HXB2 and MN) and 3 B primary viruses (BX08, CHA, 133) were amplified by PCR and inserted in the pSFV1 plasmid. After in vitro synthesis of the recombinant SFV RNA, electroporation of RNA was done on BHK-21 cells. Antigenicity was studied by RIPA and FACS using anti-HIV-1 pos. human sera and broadly neutralizing Hu Mo Abs (IgG1b12, F105, 447-52D and 2F5). Recombinant SFV particles were used to immunize mice against each envelope separately or a mixture of the 5 envelopes. Humoral responses were analyzed by both ELISA using the native form of each of these envelopes and neutralization assays.

Results: RIPA showed major antigenic differences between the 5 envelopes. The envelopes CHA and 133 were not recognized by 447-52D and IgG1b12, respectively. FACS analysis revealed that the oligomeric envelopes derived from TCLA strains were better recognized by the human sera and the monoclonal antibodies than the envelopes derived from primary isolates. The mice immunized with the mixture of envelopes raised the broadest response against the different envelopes.

Conclusion: Immunization with a mixture of envelopes presenting antigenic differences can broaden the specificity of the humoral immune response.

Keywords: AEGIS, HIV-1, T-Lymphocytes, Cell Line, Glycoproteins, Thimerosal, Edetic Acid, Cellulose, Semliki forest virus, Adapt, Human, in vitro, Animal, Mice, AIDSKWDaegis,hiv-1,t-lymphocytes,cellline,glycoproteins,thimerosal,edeticacid,cellulose,semlikiforestvirus,adapt,human,invitro,animal,mice,aids

1999-01-31
48

Copyright © 1999 - Foundation for Retrovirology and Human Health. Reproduction of this abstract (other than one copy for personal reference) must be cleared through the Foundation for Retrovirology and Human Health. Licensed from National Library of Medicine.