7th Conference on Retroviruses and Opportunistic Infections


San Francisco, CA - January 30 -February 4, 2000



COMPARISON BETWEEN IFA AND EIA ASSAYS IN DETECTING SEROCONVERSION TO HUMAN HERPESVIRUS 8 (HHV-8) IN PERSONS IDENTIFIED WITH HUMAN IMMUNODEFICIENCY VIRUS (HIV).

Conf Retroviruses Opportunistic Infect 2000 Jan 30-Feb 2;7th:75 (abstract no. 1)

T Spira, L Lam, C Pau, S Dollard, K Kite-Powell, P Pellett
CDC, Atlanta, GA

Longitudinal serum specimens from a cohort of 70 HIV-infected homosexual men were tested for antibodies to HHV-8 using two peptide EIA assays and an IFA assay for lytic antigens. The EIA assays measured antibodies to HHV-8 orf65 or K8.1 peptides. At the last time point of sampling, 35 (50%) were HHV-8 seronegative and 8 (11%) were indeterminate (above cutoff, but O.D.<0.5) by both of the peptide EIAs. Of the former, 26 (74%) were negative (≤1:40) on IFA; 8 of which were <1:10, while of the latter, 6 (75%) were negative (only 1, <1:10). Specimens taken at close to entry were then tested on those found to be EIA positive. Of these 27, 11 (41% of total) were also seropositive in the first specimen; 13 were seronegative (48%) and 2 (7%) were indeterminate. Of the 11 continuously EIA positive, all were also continuously positive on IFA. Of the 13 clear seroconvertors in the EIA, 8 (62%) also showed seroconversion in the IFA, 4 (31%) were continuously IFA positive, and 1 (8%) was IFA negative. Testing of serial specimens on the clear EIA seroconvertors showed IFA seroconversion coincident with that for EIA in about half. When IFA positivity preceded the EIA seroconversion, precedence was by 2-6 years. IFA titers over time fluctuated significantly in about half of the seroconvertors as well as in some of those continuously positive. Forty-six percent of the EIA seroconvertors and 36% of those continuously EIA positive developed Kaposi's sarcoma (KS). No EIA or IFA negative person developed KS. Median IFA titers in seroconvertors or those who were continuously EIA positive who developed KS were not significantly different from those who did not. While EIA and IFA both detected seroconversion to HHV-8, these were not always simultaneous. The significance of IFA seroconversion, with later EIA conversion, remains to be established.

Keywords: AEGiS, Herpesvirus 8, Human, HIV, Sarcoma, Kaposi, Equine Infectious Anemia, HIV Infections, Immunoenzyme Techniques, HIV Antibodies, HIV Seronegativity, Human, Male, Animal, analysis, immunologyKWDaegis,herpesvirus8,human,hiv,sarcoma,kaposi,equineinfectiousanemia,hivinfections,immunoenzymetechniques,hivantibodies,hivseronegativity,human,male,animal,analysis,immunology

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2000-01-30
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