3rd International AIDS Society Conference on HIV Pathogenesis and Treatment


Rio de Janeiro - July 24 - 27, 2005

BIOLOGICAL PHENOTYPE OF HIV-1 BG RECOMBINANTS AND OTHER GENETIC FORMS FROM GALICIA, SPAIN

IAS Conf HIV Pathog Treat 2005 Jul 24-27;3rd: Abstract No. MoOa0405

Perez-Alvarez L.1, Muñoz M.1, Delgado E.1, Casado G.1, Sierra M.1, Carmona R.1, Thomson M.1, Pérez S.2, Miralles C.3, Vázquez de Parga E.1, Ocampo A.3, Contreras G.1, Medrano L.1, Taboada J.A.4, Nájera R.1
1Viral Pathogenesis. Instituto Salud Carlos III, Majadahonda.Madrid, Spain, 2Hospital do Mexoeiro, Pontevedra, Galicia, Spain, 3Hospital Xeral Cíes, Vigo.Pontevedra, Spain, 4Conselleria de Sanidade e Servicios Sociais, Galicia, Spain

INTRODUCTION: Biological characterization and analysis of V3 loop as a determinant of viral phenotype and co-receptor use is well known in subtype B variants. In our laboratory we have obtained 40 primary isolates of non-B and recombinants HIV-1 variants. The aim of this study is to investigate the biological characteristics of different genetic forms, emphasizing the BG recombinants most frequently detected in this area, and to define the association of these properties with V3 loop sequence variability.

METHODS: 40 patients infected with different HIV-1 genetic forms were included. The most frequent were: BG recombinants (35%), including CRF14_BG (BV3) (22.5%) and BG (BV3) (12.5%), CRF02_AG (AV3) (20%), subtype G (7.5%) and subtype F (7.5%). Primary isolates (PI) were obtained by co-culture of peripheral blood mononuclear cells. Syncytium inducing (SI) phenotype was studied in MT2, and co-receptor use (CCR5 and CXCR4) in GHOST-CD4 and U87-CD4 cells. Phenotype prediction from V3 was based on basic amino acid (aa), net charge, and by the computer program http://genomiac2.ucsd.edu:8080/wetcat/v3.html (wetcat). CD4+ count and viral load level were studied.

RESULTS:

1) All BG recombinants, either CRF14_BG (BV3) or BG (BV3) strains were SI/X4, and were isolated from patients independently of the CD4+ cell count or the evolution time of the disease. Characteristic substitutions in V3 loop of these strains were observed: 13T,14M, 19V, 20W; and atypical changes in GPGR motif were detected in one BG (BV3). CRF02_AG (AV3), CRF05_DF (FV3), subtypes F, G and C were NSI/R5.

2) Prediction of co-receptor use based on the presence of basic aa at positions 11 or 25, V3 net charge and wetcat program were concordant with biological characterization of PI.

CONCLUSIONS: The biological phenotype of all BG recombinants was SI/X4, independently of the clinical stage of the disease, in contrast with subtype B. The biological phenotype of non-B subtypes and recombinants are linked to env V3 loop sequence variability.

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Basic | MoOa0405 | Mercedes Muñoz
14.2 155 14.2 HIV diversity

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