3rd International AIDS Society Conference on HIV Pathogenesis and Treatment Rio de Janeiro - July 24 - 27, 2005

HIGH DEGREE OF INTER-CLADE CROSS-REACTIVITY OF HIV-1-SPECIFIC T CELL RESPONSES ON THE SINGLE PEPTIDE LEVEL

IAS Conf HIV Pathog Treat 2005 Jul 24-27;3rd: Abstract No. TuPp0101

Yu X., Lichterfeld M., Perkins B., Chen J., Cheng M., Kang W., Brander C., Walker B., Altfeld M.
Partners AIDS Research Center, Boston, United States of America

INTRODUCTION: To determine HIV-1-specific T cell responses in clade B infected individuals recognizing the clade A, B and C consensus sequences in order to assess the degree of inter-clade cross-reactivity of these immune responses on the single epitope level.

METHODS: HIV-1-specific T cells responses were cross-sectionally assessed in 27 chronically HIV-1-infected individuals from a population mainly infected with clade B viral strains, using an interferon-γ Elispot assay with a total of 1230 overlapping peptides spanning the entire amino acid sequence of the clade A, B and C 2001 consensus sequences.

RESULTS: No significant difference was observed between the total magnitude or breadth of T cell responses recognizing either the clade A, B or C consensus sequences. However, at the single peptide level, 194 T cell responses were identified that recognized only one of the three different clade-specific peptide variants (A:B:C=34:105:55), 125 T cell responses recognized two of the three peptide variants (AB:AC:BC=71:15:39) and 166 T cell responses (34%) were cross-reactive with all three different peptide variants. Peptides recognized in all three consensus sequence variants had a significantly lower entropy (p<0.0001) and a significantly higher inter-clade homology (p<0.0001).

CONCLUSIONS: Viral epitopes within regions of low HIV-1 clade B diversity and high inter-clade homology can be recognized in the clade A, B and C variants and indicate a wide degree of cross-isolate and cross-clade recognition by HIV-1-specific T cells. These regions may therefore be of particular relevance for the design of HIV-1 vaccines.

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050724
Basic | TuPp0101 | Xu Yu
HIV-specific cellular immunity

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