4th International Workshop on Adverse Drug Reactions and Lipodystrophy in HIV 22-25 September 2002, San Diego, CA, USA

REDUCED LIPOPROTEIN LIPASE mRNA EXPRESSION IN THIGH ADIPOSE TISSUE FROM HIV-1-INFECTED PATIENTS WITH LIPOATROPHY

Antiviral Therapy 2002; 7:L3 (abstract 4)

J Chaparro, XP E, D Reeds, S Klein, CF Semenkovich, WG Powderly, K Yarasheski and E Li
Washington University-St. Louis, St. Louis, Mo., USA

OBJECTIVE/AIM: To determine if there is altered gene expression in adipose tissue collected from HIV-1-infected patients with subcutaneous lipoatrophy compared with HIV-1-infected patients without lipoatrophy.

METHODS: Subcutaneous adipose tissue was aspirated from thigh and abdomen of seven HIV-1-infected patients with thigh subcutaneous lipoatrophy and six HIV-1-infected patients with normal subcutaneous fat (as determined by MRI). Total RNA was isolated and lipoprotein lipase (LPL) and peroxisomal proliferator-activated receptor γ (PPARγ) mRNA transcript levels were measured (relative to that for β-actin) using reverse transcription and real-time fluorescence quantitation-polymerase chain reaction assays. Differences in the median ΔCT for each gene were assessed by the non-parametric Mann-Whitney U test. Differences in the mean ΔCTs between the four groups of fat biopsies were analyzed by two-way ANOVA. The threshold of significance was set at P=0.05.

RESULTS: The median LPL ΔCT values were 2.7 (abdomen) and 4.2 (thigh) for lipoatrophic subjects, and were 2.8 (abdomen) and 3.0 (thigh) for non-lipoatrophic subjects. The median PPARγ ΔCT values were 7.8 (abdomen) and 9.4 (thigh) for lipoatrophic subjects, and were 7.8 (abdomen) and 8.2 (thigh) for nonlipoatrophic subjects. The median thigh LPL ΔCT was significantly greater in lipoatrophic subjects compared to non-lipoatrophic subjects (P=0.04). The mean LPL ΔCT values from the four groups of biopsies varied significantly with respect to the presence or absence of lipoatrophy (P<0.02), but not with respect to the fat depot site (P=0.06). The median PPARγ ΔCT value was significantly greater in thigh subcutaneous fat than abdominal subcutaneous fat in lipoatrophic subjects (P=0.002). The mean PPARγ ΔCT values from the four groups varied significantly with respect to fat depot site (P=0.002) but not with respect to the presence or absence of lipoatrophy (P=0.09).

CONCLUSIONS: These preliminary results indicate that LPL mRNA expression is reduced in thigh subcutaneous adipose tissue from HIV-1-infected subjects with subcutaneous lipoatrophy.

Presenting author: E Li

2002-09-22
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