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7th Conference on Retroviruses and Opportunistic InfectionsSan Francisco, CA - January 30 -February 4, 2000 |
Conf Retroviruses Opportunistic Infect 2000 Jan 30-Feb 2; 7:76 (abstract no. 7)
N. W. Abbey, M. S. Mcgrath, And B. G. Herndier
Univ. of California, and San Francisco, San Francisco Gen. Hosp., CA
In AIDS associated macrophage (MΦ) rich lymphomas, our group observed clonal HIV inserted upstream from the c-fes proto-oncogene. These same lymphomas showed strong immunohistochemical staining in the tumor associated MΦ for the activated c-fes antibody. We used these findings to survey a variety of HIV and non-HIV associated lymphoid tissues with the hope of understanding the prevalence of upregulation of activated c-fes in lymphoid tumors. Using a standard three step immunohistochemical detection, we stained 17 samples from 16 patients with the following results.
* one patient HHV8+ w/KS and BCBL
The strongest activated c-fes staining was seen in MΦ responding to inflammation and in MΦ that were associated with MΦ rich AIDS lymphomas. Little to no staining was seen in the other AIDS lymphomas or in the non-AIDS lymphomas. Two of the three MΦ rich lymphomas have been shown to have clonal HIV inserted upstream from the c-fes proto-oncogene and the third, a BCBL, is being evaluated for insertional mutagenesis. The finding of potential upregulation of activated c-fes in HIV retroviral insertional mutagenesis has important pathologic implications.
2000-01-30
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Copyright © 2000 - Foundation for Retrovirology and Human Health (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the Foundation for Retrovirology and Human Health. Licensed from National Library of Medicine.