Objectives: Elaboration of an effective model of detection and identification of tuberculosis in AIDS patients.
Methods: 2371 samples (bronchial aspirates, blood, sputum , urine, CSF, punctates, lymph nodes, bone marrow, swabs, autopsy organ specimens) were obtained from 514 patients suspected of tuberculosis or mycobacteriosis predominantly with AIDS. The materials requiring decontamination were processed according to NALC and centrifuged at 3000 rpm/30 min. From precipitate, a preparation stained with auramin or Kinyoun were made, subjected cases to genetic AMTDT or PCR investigation (CSF, ICU, ZN +), liquid medium for diagnostic culture in the MB/ BacT AFB system and culture in L-J medium. The obtain strains were identified on the niacin test chromogenicity genetic probes for M.TB complex, MAC, M. kansasii, M. gordonae in the Accu Probe
system and with HPLC method. Drug sensitivity of M.TB complex was performed on the MB/BacT AFB system.
Results: In 74 out of 514 studied patients (14,4%) MTB complex was confirmed. Mycobacteriosis were found in 13 patients (2,7%). In eight cases M.TB complex strains were resistant to tuberculosis drugs: in seven – only to RMP and to MDR in one case.
Conclusions: Application of genetics methods and fast diagnosis culture systems significantly improved the laboratory diagnosis compared with the conventional L-J cultures.
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